Ultra and High Speed Centrifugation
Super/Ultra Centrifuges and Rotors available for biomolecule separation, subcellular fraction separation, density gradient separation, and pelletisation of bacteria and cells.
Before using the instruments for the first time, contact the trainers below for mandatory training.
Users are expected to perform experiments independently and must be adequately trained to ensure proper use of the instruments.
How to make reservation
Prior to use, it is mandatory to reserve instruments through the booking platform of the Department of Biomedical Sciences.
If you don’t have an account to access the booking platform, please contact our ICT service staff at ‘informatica.biomed@unipd.it’.
Coordination & Training
Service coordinator
TOTTENE ANGELITA
Training & support staff
CESARO LUCATREVISAN ELENACHIAVEGATO ANGELAFOLDA ALESSANDRA
The Facility’s staff are available Monday to Friday during normal working hours (09:00-16:00).
Where is & How the facility works
Location
Room 2-124 (floor 1N), Vallisneri building
Room 6-126 (floor 5N), Vallisneri building
Room 3-135 (floor 2N), Vallisneri building
Room 6-136 (floor 5N), Vallisneri building
Room 5-126 (floor 4N), Vallisneri building
Opening and closing
Monday - Sunday | 24h
It is possible to reserve time slots that are at least 15 minutes long. The rate is determined by the booking time.
For information: ultracentrifugation.biomed@unipd.it
Facility equipment
Room 6-136 (floor 5N), Vallisneri building
centrifugeBeckman Avanti J25
Available Manuals:
Room 5-126 (floor 4N), Vallisneri building
centrifugeBeckman Avanti J25
Available Manuals:
Room 2-124 (floor 1N), Vallisneri building
centrifugeBeckman L70
Available Manuals:
Room 6-126 (floor 5N), Vallisneri building
centrifugeBeckman Optima L-90K
Available Manuals:
Room 3-135 (floor 2N), Vallisneri building
centrifugeBeckmann Optima Max-XP
Available Manuals:
Room 3-135 (floor 2N), Vallisneri building
centrifugeEppendorf 5810R
Available Manuals:
Room 3-135 (floor 2N), Vallisneri building
centrifugeHITACHI CR22N
Available Manuals:
Room 2-124 (floor 1N), Vallisneri building
centrifugeSorvall LYNX 4000
Available Manuals:Before your session
Wear clean gloves to pick up the rotors and set the centrifuges.
It’s forbidden to make any intervention on machine or its electric system.
It’s forbidden to centrifuge pathogenic organism and biological material which may be hazardous.
The system must be checked in all its parts, must be clean and without any obvious damage; if this is not the case, please contact the staff immediately.
Tubes must be safely and firmly closed. Tubes must be filled following the volume indications in the manufacturer’s manual.
Buckets and tubes must be correctly balanced. Even if you don’t need all of them, please use swinging rotors with all buckets.
During your session
Never exceed 80% of the maximum operating speed of the rotor.
Please wait until the rotor reaches the set speed before leaving the room.
If there are vibrations or anomalous noises, immediately stop the run and call Service staff.
In case of damaged tubes and loss of material during the run, stop it and wait at least 30 minutes to allow the deposition of aerosol particulates. All damaged tubes, glasses fragments, accessories and rotor must be submerged into ethanol 70% for 24h. Throw them away as biological wastes. Please wear appropriate DPI for all operations.
After your session
Switch the centrifuge off; remember to leave the lid open, to allow the chamber to dry. The rotors have to be cleaned and put back in the cold rooms of the corresponding floor.
Malfunction or problems on centrifuges before, during or after use have to be notified to the Service Staff.
Specific indication for this facility
Prior to use, it is mandatory to reserve instruments through the booking platform of the Department of Biomedical Sciences, at https://prenotazioni.biomed.unipd.it/strumenti/week.php, using your laboratory’s account. If you don’t have an account to access the booking platform, please contact our ICT service staff at ‘informatica.biomed@unipd.it’.
When making your reservation, indicate the name of the user, the group leader, and lab phone number.
If necessary, reservations can be cancelled no later than 24h prior to the reserved slot.
