Tissue processing and Histology
The tissue processing facility @DSB provides an essential platform for the preparation of tissue samples used in biomedical research. Its instruments, such as cryostats, microtome, tissue dissociators and homogenizers, automated tissue processors, and paraffin embedding stations, provide users with an efficient and specialized environment for the preparation and analysis of tissue samples, enabling in-depth research in the fields of cell biology, pathology, and tissue engineering.
Before using the instruments for the first time, contact the trainers below for mandatory training.
Users are expected to perform experiments independently and must be adequately trained to ensure proper use of the instruments.
How to make reservation
Prior to use, it is mandatory to reserve instruments through the booking platform of the Department of Biomedical Sciences.
If you don’t have an account to access the booking platform, please contact our ICT service staff at ‘informatica.biomed@unipd.it’.
Coordination & Training
Service coordinator
MOLON BARBARA
Training & support staff
CHIAVEGATO ANGELACACCIN PAOLABONDI' MICHELA
The Facility’s staff is available Monday to Friday during working hours (09:00-16:00).
Where is & How the facility works
Location
Room 2-132 (floor 1N), Vallisneri building
Room 02-120 (floor 1N), Vallisneri building
Room 2-106 (floor 1N), Vallisneri building
Opening and closing
Monday - Friday | from: 08:00 to: 19:00
Please note: the Facility is closed in the closing days of the Department.
For information: tissueprocessing.biomed@unipd.it
Facility equipment
Room 2-132 (floor 1N), Vallisneri building
cryostatLeica CM1850 Cryostat
Available Manuals:
Room 2-132 (floor 1N), Vallisneri building
cryostatLeica CM1950 Cryostat
Available Manuals:
Room 2-106 (floor 1N), Vallisneri building
dissociator / tissue processorTissue Lyser Qiagen
No manuals available for this instrument.
Before your session
- Wear clean gloves
- Equipments must be checked in all their parts: everything must be clean and without any obvious damage. If this is not the case, please immediately contact the staff.
- Report to the staff the presence of incorrect behavior or waste not properly disposed of.
During your session
- The cutting wheel must always be locked when handling samples inside the cryostat.
- The cryostat window must not be left open unnecessarily in order to prevent condensation formation
After your session
- DO NOT leave anything in the room and inside the cryostat.
- After use, it is RECOMMENDED to accurately CLEAN and DISINFECT the instrument and surfaces:
for CM1850 clean inside ONLY with Ethanol 90-100%
for CM1950 clean inside ONLY with Ethanol 90-100% and turn on UV (15’cycle during the day and 45’ after the last session of the day)
- At the end of your session, return the cryostat to its standby temperature (-20°C). Collect any biological waste in a resealable plastic bag (provided by the Facility) and throw it in the organic black bin or take it back to your lab and dispose of it appropriately.
Specific indication for this facility
The Facility does not provide accessories such as brushes, tweezers, blades, OCT, anti-roll system to internal (DSB) users. A set of accessories is available for the exclusive use of external users.
Università degli Studi di Padova
Dipartimento di Scienze Biomediche
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