HOOD n.2 - Bioair AURA HZ48

1. GENERAL

1. Upon completion of the training (mandatory), you will be added to a mailing list for urgent notifications.
2. The access to the room is allowed upon reservation only (see “Booking procedures” section above)
3. In the room, the use of lab coats and gloves is mandatory. Please tie long hair.
4. Eating, drinking and storing food is forbidden inside the room.
5. All equipment has to be kept clean and switched off after use.
6. In case of problems/instrumentation malfunction, please contact the staff immediately

2. SPACES AND EQUIPMENTS

1. SPACES
   For the allocation of space (workbenches, fridge/freezer), please contact the staff. Assigned space allocation must be respected. Stored materials should be marked with the name of the owner laboratory. At the end of your turn, all materials must be returned to their assigned spaces.
   DO NOT leave anything in the hood and on the common shelves/working benches.
2. HOODS
   Hoods are shared, and must be left completely empty at the end of each working session. Pipettes and pipettors are provided by the users. During your turn, please remember to keep minimal equipment under the hood, to avoid laminar airflow block. If leakage occurs, the staff must be informed and the hood work surface MUST BE CLEAN immediately. If the leakage spreads under the inner steel surface of the hood, it must be removed and the surface below cleaned.
   At the end of the working session, each user MUST CLEAN the work surface and glass shield with 70% alcohol, and switch hood UV lamp on for 20 min. Please remember to also consider this final hood UV sterilization time when you book your slot. UV lamps of the hoods do not have an automatic timer. You must SWITCH ON/OFF the UV lamp by plugging/unplugging it into/from the socket. Remember to check that the UV lamp is switched off at the end of the sterilization cycle.
3. WATER BATH
   The water bath available in the room is filled with distilled water. Before using it, please CHECK the water level. Remember to SWITCH IT OFF AFTER USE.
4. VACUUM PUMP
   Prior to starting aspiration, each user should attach a sterile pipette tip or pasteur pipette to the end of the aspiration hose. At the end of the work, all users are required to sanitize the aspiration junction by aspirating bleach, followed by H2O; moreover, the waste collection tank has to be emptied at the end of each turn.
5. INCUBATORS
   The facility is equipped with:
   • a Department incubator, available for shared use upon booking through the Department website. Flasks/plates in this incubator have to be marked with the name of the user and the lab.
   • private laboratory incubators (up to 4 positions available), accessible upon request
   
   In both cases, cleaning, management of the incubator and gas tank replacement are charged to the user. Current user contacts have to be specified on the incubator door.
   If not used, incubators should be turned off; a sterilization cycle has to be done once restarted, prior to use.
6. MICROSCOPE
   The Leica Mateo TL provided is equipped with 4x, 10x, 20x and 40x objectives. The microscope allows users to capture images of cell cultures. Please export all images immediately at the end of your session with a USB key. The memory will be emptied every Monday from the staff of the facility.
   At the end of your session, TURN OFF the lamp and leave the microscope objective and plate CLEAN.
   For the first use, please contact the facility staff.

Inspections of spaces and equipment are regularly carried out by the Facility and the Department Staff or by qualified personnel, but ORDINARY MAINTENANCE MUST BE CARRIED OUT BY ALL USERS.

3. WASTES
Each user is in charge of waste management. During the period of use, one solid biological bin (black; with a lid on it) and one tank for liquid biological wastes per lab can be kept in the room. Both containers must be labelled with the name of the owner laboratory. Once full, or in the event of an extended period of inactivity in the room, containers must be sealed and handed over by the user to the Safety service for proper disposal.

4. IN CASE OF BIOLOGICAL CONTAMINATIONS IN THE ROOM
In case of fungal or bacterial contaminations, it is necessary to strictly follow the protocol below:

1. inform the staff immediately
2. decontaminate contaminated plates, flasks, shelves and equipment used. Specifically, add hypochlorite to the plates and flasks, agitate, incubate approximately 10 minutes, then pour the solution into the liquid waste tank
3. Preferentially dispose of, or carefully inspect, all materials used (culture media, trypsin, PBS, plastics, etc.)
4. Thoroughly clean the incubator. If the contamination regards several flasks/plates, consult with the staff to initiate a sterilization cycle
5. Deeply clean the biosafety hood used, including sterilization for at least 30 minutes
6. immediately remove all your solid and liquid wastes from the room; at the end of the procedure, SWITCH ON the room’s UV lamp for a complete disinfection cycle for 20 minutes. Please notify the staff so they can inform other users that access to the room is temporarily forbidden.