Seahorse XFe24
flux analyzer of Seahorse facility
24 wells flux analyzer (Seahorse Biosciences)
Room 3-081 (floor 2N), Vallisneri building
Monday - Friday | 24h
The calendar is divided in 3.5 hours/session, for single experiments:
- Session 1 (09:00 – 12:30);
- Session 2 (12:30 – 16:00);
- Session 3 (16:00 – 19:30);
- Session 4 (19:30 – overnight).
PAY ATTENTION! Instruments cannot be used during the weekend; at the end of your session, please check if you are the last user of the week and, in this case, switch it off.
BOOK NOWService coordinator
DE STEFANI DIEGO
Training & support staff
TREVISAN ELENA
User support material:
- Part 1: Agilent Seahorse XF Training Webinar: Gather Assay Materials | Agilent
- Part 2: Agilent Seahorse XF Training Webinar: Prepare for your XF assay | Agilent
- Part 3: Cell Analysis Training - Set Up to Run XF Assays | Agilent
- How to run an assay | Agilent
For specific technical assistance, please feel free to contact Lorena Maria Ferreira from AHSI (ferreira@ahsi.it).
Specific indication for the facility
ATTENTION PLEASE: Only Agilent Technologies consumables (plates and kits) are compatible with XF analyzers; they are not included in the service.
Below the "Seahorse consumables list":
Before your session
The system must be checked in all its parts, must be clean and without any obvious damage; if this is not the case, please contact immediately the staff.
After your session
- Always remove or transfer your files. Staff will periodically remove the user files without prior notice.
- The default set temperature of measurement is 37°C; if changed to perform specific experiments, it has to be set again at 37°C at the end of the session (see “temperature control”).
- Please check if you are the last user of the week and, in this case, switch the instrument off.
How to make reservation
Prior to use, it is mandatory to reserve instruments through the booking platform of the Department of Biomedical Sciences.
If you don’t have an account to access the booking platform, please contact our ICT service staff at ‘informatica.biomed@unipd.it’.




