FACILITY

Seahorse

Seahorse analyzers are live-cell metabolic assay platforms that allow to measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in 24/96 wells plates, interrogating key cellular functions such as mitochondrial respiration and glycolysis. They perform compounds addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.

Before using the instruments for the first time, contact the trainers below for mandatory training.
Users are expected to perform experiments independently and must be adequately trained to ensure proper use of the instruments.

How to make reservation

Prior to use, it is mandatory to reserve instruments through the booking platform of the Department of Biomedical Sciences.

If you don’t have an account to access the booking platform, please contact our ICT service staff at ‘informatica.biomed@unipd.it’.

Coordination & Training

Service coordinator

DE STEFANI DIEGO

Training & support staff

TREVISAN ELENA (elena.trevisan@unipd.it)

User support material:

For specific technical assistance, please feel free to contact Lorena Maria Ferreira from AHSI (ferreira@ahsi.it).

Where is & How the facility works

Location

Room 3-081 (floor 2N), Vallisneri building

Opening and closing

Monday - Friday | 24h

The calendar is divided in 3.5 hours/session, for single experiments:

Session 1 (09:00 – 12:30);
Session 2 (12:30 – 16:00);
Session 3 (16:00 – 19:30);
Session 4 (19:30 – overnight).

PAY ATTENTION! Instruments cannot be used during the weekend; at the end of your session, please check if you are the last user of the week and, in this case, switch it off.

Fees

For information write to: seahorse.biomed@unipd.it

Facility strumentation

Book now

^{Room 3-081 (floor 2N), Vallisneri building}

_{flux analyzer}

Seahorse XF PRO

Available Manuals:

Book now

^{Room 3-081 (floor 2N), Vallisneri building}

_{flux analyzer}

Seahorse XFe24

Available Manuals:

Before your session

The system must be checked in all its parts, must be clean and without any obvious damage; if this is not the case, please contact immediately the staff.

After your session

Always remove or transfer your files. Staff will periodically remove the user files without prior notice.
The default set temperature of measurement is 37°C; if changed to perform specific experiments, it has to be set again at 37°C at the end of the session (see “temperature control” below).
Please check if you are the last user of the week and, in this case, switch the instrument off.

Specific indication for this facility

ATTENTION PLEASE: Only Agilent Technologies consumables (plates and kits) are compatible with XF analyzers; they are not included in the service.

Below the "Seahorse consumables list":

Seahorse XFe24

Seahorse XF PRO